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Nanoparticle Tracking Analysis (NTA)
Size range
10 – 1000nm (sample and laser dependent)
Concentration range
10⁵ – 10⁹ particles/ml
Sensitivity level
Fluorescence < 20AF488 molecules
Zeta potential working range
-500mV – +500mV
C-NTA
Colocalization of two fluorophores on one particle
Measurement specifications
Size
- 10 – 1000nm (dependent on sample and laser)
- Accuracy: ±5% (for 100nm polystyrene latex)
Concentration
- Concentration range: 10⁵ – 10⁹ particles/ml
- Accuracy: ±5% (for 100nm polystyrene latex)
Fluorescence
- Concentration range: 10⁵ – 10⁹ particles/ml
- Sensitivity level: < 20AF488 molecules
Zeta potential*
- Working range: -500mV – +500mV
- Concentration range: 10⁶ – 10¹⁰ particles/ml
- Conductivity range: 3µS/cm – 15mS/cm
- Reproducibility: ±2mV (zeta potential standard)
General
- Minimum sample quantity: 500µl of sample at 10⁵ particles/ml
- pH range: 1 – 13
Reference material
- Nominal 100nm size and concentration reference suspension
- Four nominal 100nm and 200nm reference suspensions for fluorescence
- Nominal -50mV reference suspension for zeta potential*
AF2000 MultiFlow Series
Universal Particle/Polymer/Protein Separator
Asymmetric flow field-flow fractionation (AF4) is an innovative new separation method for the efficient separation and characterization of proteins, polymers and nanoparticles in a fast and gentle way.
- Separation in a narrow ribbon-like channel
- Cross flow as separation field perpendicular to the solvent flow
- Separation according to different diffusion coefficients (hydrodynamic size)
- Applicable particle size range from 1 nm up to a few μm
- Polymers
- Nanoparticles
- Polymersomes
- Proteins
- Viruses
- Rubbers
- Polysaccharides
- Liposomes
- Antibodies
- Exosomes
Polymers in solution and/or in suspension
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